葡萄糖对三角褐指藻生长、岩藻黄素含量及相关基因表达的影响

刘浩，杭雨晴，朱帅旗，王何瑜，龚一富

中国药学杂志 ›› 2016, Vol. 51 ›› Issue (14) : 1230-1234.

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中国药学杂志
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中国药学杂志 ›› 2016, Vol. 51 ›› Issue (14) : 1230-1234. DOI: 10.11669/cpj.2016.14.017
论著

葡萄糖对三角褐指藻生长、岩藻黄素含量及相关基因表达的影响

  • 刘浩，杭雨晴，朱帅旗，王何瑜，龚一富*
作者信息 +

Effects of Glucose on the Growth of Phaeodactylum tricornutum, Fucoxanthin Content and Related Gene Expression

  • LIU Hao, HANG Yu-qing, ZHU Shuai-qi, WANG He-yu, GONG Yi-fu*
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文章历史 +

摘要

目的 研究葡萄糖对三角褐指藻细胞生长、岩藻黄素含量和岩藻黄素生物合成相关基因表达的影响。方法 本实验以分光光度法研究葡萄糖对三角褐指藻生长影响，HPLC研究藻细胞岩藻黄素含量，荧光定量聚合酶链式反应(PCR)研究岩藻黄素生物合成相关基因表达。结果 在平台期末，葡萄糖处理组藻细胞密度均比对照组高，说明葡萄糖促进三角褐指藻细胞生长。HPLC结果表明，随着葡萄糖浓度的升高，三角褐指藻细胞中岩藻黄素含量呈逐渐下降的趋势。其中，葡萄糖为50 mg·L-1时，岩藻黄素含量最低，为0.26 mg·g -1干重， 比对照组降低了67.5%，说明葡萄糖抑制了三角褐指藻岩藻黄素的生物合成。荧光定量PCR结果表明，10~50 mg·L-1葡萄糖处理三角褐指藻时，岩藻黄素生物合成相关基因zep、pys、zds、lcyb、crtiso和pds的表达量均比对照组低。该结果与岩藻黄素含量变化一致。结论 葡萄糖抑制三角褐指藻岩藻黄素的生物合成是通过下调岩藻黄素生物合成相关基因表达来实现的。

Abstract

OBJECTIVE To explore the effects of glucose on the growth of algae, the content of fucoxanthin, and expressions of genes related to biosynthesis of fucoxanthin. METHODS The cell growth of algae induced by glucose was researched by using spectrophotometric method. The content of fucoxanthin was measured by HPLC. The expressions of the genes which were related to biosynthesis of fucoxanthin were detected by using quantitative PCR. RESULTS At the end of the platform period, the density of cells treated by glucose was higher than that of the control group. The growth of P. tricornutum was promoted by glucose. The result of HPLC analysis showed that fucoxanthin content in the algae treated by different concentrations of glucose was decreased than that in the control group. When the concentration of glucose reached 50 mg·L-1, the content of fucoxanthin was the lowest (0.26 mg·g-1 DW), which was 67.5% lower than the control, indicating that glucose inhibited the biosynthesis of fucoxanthin in P. tricornutum. RT-qPCR result showed that the expression of genes related to the biosynthesis pathway of fucoxanthin, i.e., zep, pys, zds, lcyb, crtiso, and pds, were all lower than those of the control when the concentration of glucose was in the range from 10 to 50 mg·L-1. This result was consistent with the change of fucoxanthin content. CONCLUSION This result further illustrates that glucose may inhibit the biosynthesis of fucoxanthin in P. tricornutum by down-regulating the expression of related genes.

关键词

葡萄糖 / 三角褐指藻 / 生长 / 岩藻黄素 / 基因表达

Key words

glucose / P. tricornutum / growth / fucoxanthin / gene expression

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刘浩，杭雨晴，朱帅旗，王何瑜，龚一富. 葡萄糖对三角褐指藻生长、岩藻黄素含量及相关基因表达的影响[J]. 中国药学杂志, 2016, 51(14): 1230-1234 https://doi.org/10.11669/cpj.2016.14.017
LIU Hao, HANG Yu-qing, ZHU Shuai-qi, WANG He-yu, GONG Yi-fu. Effects of Glucose on the Growth of Phaeodactylum tricornutum, Fucoxanthin Content and Related Gene Expression[J]. Chinese Pharmaceutical Journal, 2016, 51(14): 1230-1234 https://doi.org/10.11669/cpj.2016.14.017
中图分类号： R284   

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基金

浙江省科技厅重点科技创新团队资助项目(2012R10029-07,2010R50029);宁波科技攻关项目(2013C10018);宁波市农村创新创业项目(2014C91023);浙江省大学生科技创新活动计划暨新苗人才计划资助项目(2014R405050);宁波大学优秀学位论文培育基金资助项目(py2014020)
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